Coding

Part:BBa_K1985009:Design

Designed by: Braulio Carrillo-Sanchez, Jasmine Cornish   Group: iGEM16_Kent   (2016-10-14)


AraC pBAD mamOPXT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2081
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4856
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1946
    Illegal AgeI site found at 2030
    Illegal AgeI site found at 2939
    Illegal AgeI site found at 3600
    Illegal AgeI site found at 4169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2561
    Illegal BsaI.rc site found at 1232
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 4736


Design Notes

Codon optimised


Source

BBa_K1321333, BBa_K1985006, BBa_K1985000, BBa_K1985001 and BBa_K1985002

References